Journal: BMC Cardiovascular Disorders
Article Title: Proteome-scale profiling reveals MAFF and MAFG as two novel key transcription factors involved in palmitic acid-induced umbilical vein endothelial cell apoptosis
doi: 10.1186/s12872-021-02246-5
Figure Lengend Snippet: Alleviation of early apoptosis induced by PA in SV40T-transformed HUVECs overexpressing MAFF and MAFG. a RT-qPCR validation of MAFF OE. b RT-qPCR validation of MAFG OE. c WB validation of MAFF OE. d WB validation of MAFG OE. e–h The first apoptosis assay by flow cytometry. i – l The second apoptosis assay by flow cytometry. m – p the third time of apoptosis assay by flow cytometry. e , i , m Control-500 μM PA group subjected to three independent apoptosis assays by flow cytometry. f , j , n Plasmid-500 μM PA group subjected to three independent apoptosis assays by flow cytometry. g , k , o Results for the MAFF OE-500 μM PA group in the three independent apoptosis assays by flow cytometry. h , l , p Results for the MAFG OE-500 μM PA group in the three independent apoptosis assays by flow cytometry. q Histogram summary results in the four groups. The dots located in Q4 represent early apoptotic cells. OE represents overexpression. ** indicates 0.001 < P < 0.01 and *** indicates P < 0.001. Control-500 μM PA: without transfection and with 500 μM PA stimulation. Plasmid-500 μM PA: with empty plasmid transfection and with 500 μM PA stimulation. MAFF OE-500 μM PA: MAFF OE plasmid transfection and 500 μM PA stimulation. MAFG OE-500 μM PA: MAFG OE plasmid transfection and 500 μM PA stimulation
Article Snippet: SV40T-transformed HUVECs were purchased from ProCell company (catalogue no. CL-0675, Wuhan, China).
Techniques: Transformation Assay, Quantitative RT-PCR, Apoptosis Assay, Flow Cytometry, Plasmid Preparation, Over Expression, Transfection
